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Index one: the i7 index column is during the forward orientation (match the orientation In case the i7 indexes during the sample sheet).
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Established D Index Adapters Check with Index 2 (i5) Orientation To find out more on how to enter i5 bases about the sample sheet in ahead or reverse complement orientation.
For Neighborhood Run Manager (LRM), input the sequences in the reverse complement when putting together the run so the computer software routinely produces the ahead orientation as required for analysis. This is often the alternative of nearly all of our steering which is only for the MiniSeq Swift kits.
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Runs uploaded in manual mode have their data directly handed to bcl2fastq without having further more processing.
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When evaluating index sets for coloration balance, the sequence observed by the instrument has to be assessed, whatever the orientation useful for the sample sheet. For the i7 index, often asses colour harmony utilizing the "i7 bases for sample sheet" column orientation.
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